维也纳医科大学临床胸外科的Hendrik Jan Ankersmit研究小组成功表明，辐射白细胞释放物质可减少严重性心脏病或中风和脊髓损伤造成的损害，而且对组织修复有积极的作用。然而，到目前为止还不知道哪些特定物质会产生有利影响。
该研究是由Ankersmit 和Michael Mildner (维也纳医科大学皮肤病学系)指导，作为Lucien Beer博士论文的一部分而出现的，他是维也纳医科大学放射学和核医学的助理医生，相关文章已经发表在《Scientific Reports》杂志上。
该研究的主要发现：“经纯化的外来体或蛋白质片段会产生有利影响。”Ankersmit解释道。除了这些蛋白复合物，脂质沉积(含脂肪物质)和其它微粒也参与其中。电离辐射刺激释放更多的这种混合物，也使我们能够调节它所包含的物质的特性。通过这种方式，白细胞可以作为生物反应器生产这些物质，称为APOSEC(分泌蛋白质组细胞凋亡缩写)。这些生物反应器很容易取得。
研究人员使用人类灭活病毒的APOSEC，奥地利食品安全机构(AGEs)已经被批准用于人类临床试验，并且它非常类似于测试通过AGEs批准的产品。大型动物模型所示(与维也纳医科大学心脏科合作)可以显著减少心脏病发作的损伤。Ankersmit说：“这些积极的发现对我们人类皮肤和心脏病研究领域给予了希望。”
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Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration
Lucian Beer, Matthias Zimmermann, Andreas Mitterbauer, Adolf Ellinger, Florian Gruber, Marie-Sophie Narzt, Maria Zellner, Mariann Gyöngyösi, Sibylle Madlener, Elisabeth Simader, Christian Gabriel, Michael Mildner & Hendrik Jan Ankersmit
Abstract We previously showed that, when peripheral blood mononuclear cells (PBMCs) were stressed with ionizing radiation, they released paracrine factors that showed regenerative capacity in vitro and in vivo. This study aimed to characterize the secretome of PBMCs and to investigate its biologically active components in vitro and vivo. Bioinformatics analysis revealed that irradiated PBMCs differentially expressed genes that encoded secreted proteins. These genes were primarily involved in (a) pro-angiogenic and regenerative pathways and (b) the generation of oxidized phospholipids with known pro-angiogenic and inflammation-modulating properties. Subsequently, in vitro assays showed that the exosome and protein fractions of irradiated and non-irradiated PBMC secretome were the major biological components that enhanced cell mobility; conversely, secreted lipids and microparticles had no effects. We tested a viral-cleared PBMC secretome, prepared according to good manufacturing practice (GMP), in a porcine model of closed chest, acute myocardial infarction. We found that the potency for preventing ventricular remodeling was similar with the GMP-compliant and experimentally-prepared PBMC secretomes. Our results indicate that irradiation modulates the release of proteins, lipid-mediators and extracellular vesicles from human PBMCs. In addition our findings implicate the use of secretome fractions as valuable material for the development of cell-free therapies in regenerative medicine.